Thermo Fisher Scientific HA Tag Monoclonal Antibody (5B1D10)
HA 태그가 결합된 융합 단백질 검출용 마우스 단클론 항체로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며, 단백질 위치 확인 및 상호작용 연구에 유용. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | 21 publications |
| Immunohistochemistry (IHC) | – | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 1 publication |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | 8 publications |
| ELISA | 0.1–1.0 µg/mL | – |
| Immunoprecipitation (IP) | – | 4 publications |
| Miscellaneous PubMed (Misc) | – | 3 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B1D10 |
| Immunogen | Synthetic peptide corresponding to the nine amino acid sequence HA-tag (YPYDVPDYA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533092 |
Product Specific Information
This monoclonal antibody reacts specifically with HA-tagged fusion proteins. Reactivity with various HA-tagged fusion proteins has been confirmed by western blotting; positive control used were HA-tagged JNK-1 transfected cell lysates.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag used to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize low abundance or poorly immunogenic proteins when specific antibodies are unavailable. HA (hemagglutinin) is derived from influenza hemagglutinin and is commonly expressed as a tag to aid identification and purification of tagged proteins for functional analysis. HA tag antibodies facilitate protein detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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