Thermo Fisher Scientific HA Tag Monoclonal Antibody (5B1D10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 21 publications 21 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 8 publications 8 publications

ELISA (ELISA)

0.1-1.0 µg/mL

-

Immunoprecipitation (IP)

-

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5B1D10

Immunogen

A synthetic peptide corresponding to the nine amino acid sequence HA-tag (YPYDVPDYA).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533092

Product Specific Information

This monoclonal antibody reacts specifically with HA-tagged fusion proteins and reactivity with various HA-tagged fusion proteins has been confirmed by western blotting; positive control used were HA-tagged JNK-1 transfected cell lysates.

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.