Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (24-31), PerCP-eFluor 710, eBioscience
인간 및 사이노몰거스 원숭이 CD154(CD40 Ligand)을 인식하는 단클론항체로, PerCP-eFluor 710으로 표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 488 nm 레이저로 여기되어 710 nm에서 방출됩니다. 항원제시세포와의 상호작용 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Cynomolgus monkey, Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 24-31 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482/708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10670357 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Request custom conjugation
Product Specific Information
Description:
The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen-presenting cells (B cells, monocytes/macrophages, dendritic cells), CD154 plays a crucial role in T-APC interaction. CD154-CD40 binding induces T-dependent B-cell activation and B-cell cycle entry. The 24-31 clone cross-reacts with cynomolgus monkey CD154.
Applications Reported:
This antibody is reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometry using stimulated normal human peripheral blood cells. Recommended use: 5 µL (0.06 µg) per test in 100 µL final volume. Suitable for 10⁵–10⁸ cells/test.
Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.
- Excitation: 488 nm (Blue Laser)
- Emission: 710 nm
- Recommended filters: 685 LP dichroic mirror and 710/40 band pass filter (695/40 acceptable)
PerCP-eFluor® 710 conjugated antibodies are stable after overnight exposure to 2% formaldehyde at 4°C. Evaluate for alternative fixation protocols as needed.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD40 Ligand (CD40-L, CD154) is a membrane glycoprotein expressed on T cells. It stimulates B cell proliferation and immunoglobulin secretion in the presence of cytokines. CD40 Ligand also induces cytokine production and tumoricidal activity in monocytes and co-stimulates T cell proliferation with IFN-γ, TNF-α, and IL-2 production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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