Thermo Fisher Scientific Rat VAMP2 (aa 1-18) Synthetic Peptide

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species Reactivity

Rat

Class

Synthetic

Type

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

purified

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

PEP-101 is a 18 amino acid synthetic peptide whose sequence corresponds to amino acid residues 1-18 of rat VAMP-2. The sequence of this peptide is (amino to carboxy terminus): M (1)-S-A-T-A-A-T-V-P-P-A-A-P-A-G-E-G-G (18)

This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and rat VAMP-2, catalog # PA1-766. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~70-fold) for competitive inhibition of antibody-protein binding reactions.

Reconstitute with 0.1 mL of distilled water.

Target Information

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer`s disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.