Thermo Fisher Scientific CD64 Monoclonal Antibody (X54-5/7.1), PE, eBioscience
마우스 CD64(FcγRI) 인식용 단클론 항체로, PE 형광체가 결합된 제품입니다. Flow cytometry 및 ICC/IF 분석에 적합하며, 0.5 µg/test로 최적화되었습니다. 다양한 마우스 계통에서 반응하며, 높은 특이성과 안정성을 제공합니다.
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Thermo Fisher Scientific CD64 Monoclonal Antibody (X54-5/7.1), PE, eBioscience™
Applications and Tested Dilution
- Immunocytochemistry (ICC/IF): Not specified
- Flow Cytometry (Flow): 0.5 µg/test
Publications
- ICC/IF: View 1 publication
- Flow Cytometry: View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | X54-5/7.1 |
| Immunogen | BALB/c mouse CD64-human IgG Fc fusion protein |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735014 |
Additional Formats
- APC
- APC-eFluor 780
- PerCP-eFluor 710
- Custom conjugation available on request
Product Specific Information
This X54-5/7.1 monoclonal antibody recognizes allelic variants a and b of mouse CD64 (FcγRI).
- Alloform a: C57BL/6, BALB/c, DBA/2
- Alloform b: C3H/HeJ, CBA/J, NZW, SJL/J, 129/SvJ
- Also reported positive in strains: AKR, ALR, BUB, C58, CE, HRS, MRL, MON, NZB, NZO, NZW, PL, SJL, ST, SWR
- Not reactive with allelic variant d (NOD/Lt mice)
- Stains RAW264.7 cell line
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse bone marrow cells (≤ 0.5 µg/test; 100 µL final volume)
Recommended careful titration for optimal assay performance.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
CD64 (FCGR1A) encodes a high-affinity Fc-gamma receptor involved in immune response.
Located on chromosome 1, it is one of three related gene family members.
Dysfunction of CD64 has been associated with cervical adenitis and tetrasomy 21.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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