Takara SMART-Seq v4 3` DE Kit—simplified high-performance single-cell differential expression analysis

Single-cell transcriptomics is important in understanding how gene expression of individual cells varies within cell populations. We have developed an end-capture method that focuses the majority of sequencing data on the 3ends of mRNA transcripts from single cells. This enables you to collect high quality data with fewer sequencing reads. This kit incorporates features from the industry-leading SMART-Seq v4 kit, including SMART (**S**witching **M**echanism **a**t 5 End of RNA Template) and locked nucleic acid (LNA) technology.

Single-cell transcriptomics is important in understanding how gene expression of individual cells varies within cell populations. We have developed an end-capture method that focuses the majority of sequencing data on the 3ends of mRNA transcripts from single cells. This enables you to collect high quality data with fewer sequencing reads. This kit incorporates features from the industry-leading SMART-Seq v4 kit, including SMART (**S**witching **M**echanism **a**t 5 End of RNA Template) and locked nucleic acid (LNA) technology.

In addition to focusing reads on the 3` end of mRNA transcripts, this kit also includes in-line indexes that serve as cell barcodes on Read 2 that enable pooling of up to 12 samples per Illumina index pair, resulting in up to 1,152 samples per sequencing lane. This pooling further reduces the time and costs of discovery. This kit takes a convenient input volume of 1–10.5 µl and relies on the Illumina® Nextera® XT DNA Library Preparation Kit. This kit has been designed and validated to prepare cDNA samples and subsequent sequencing libraries for the following Illumina sequencing platforms: HiSeq®, NextSeq®, MiSeq®, and MiniSeq™.

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Overview

• Improved SMART-technology-based chemistry—SMART-Seq v4 oligo with LNA technology plus improved formulations lead to better performance (high sensitivity, better reproducibility, and more GC-rich genes detected).
• End-capture protocol—the majority of reads are focused on the 3` end of samples while maintaining excellent sensitivity, reducing the number of reads required for differential expression analysis. More information can be found in this tech note.
• Pooling of up to 12 samples per Illumina index pair—in-line indexes serve as cell barcodes and enable pooling of up to 12 samples per Illumina index pair (1,152 samples per sequencing lane), further reducing time and costs.

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Applications

• cDNA synthesis for end-capture mRNA-seq for differential expression analysis of single cells

Interested in more data and FAQs about this product? Visit the NGS Learning Center.

If you are looking for software to demultiplex your sequencing data generated with this kit, please visit the SMART-Seq DE3 Demultiplexer webpage.

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.