Takara Cellartis 2i mES/iPSC Culture Medium: maintenance of germline-competent mouse ES and iPS cells

Cellartis 2i mES/iPSC Culture Medium is a serum-free, defined cell culture medium for the establishment of mouse ES and iPS cell lines. This medium contains two selective small molecule inhibitors (2i technology: CHIR99021 and PD0325901) that act to eliminate differentiation-inducing signals from GSK3β and ERK/MEK, respectively, and promote cell proliferation.

Cellartis 2i mES/iPSC Culture Medium is a serum-free, defined cell culture medium for the establishment of mouse ES and iPS cell lines. This medium contains two selective small molecule inhibitors (2i technology: CHIR99021 and PD0325901) that act to eliminate differentiation-inducing signals from GSK3β and ERK/MEK, respectively, and promote cell proliferation.

The culture medium alone can be used for long-term maintenance of naive, ground state mouse embryonic stem cells or fully pluripotent mouse iPS cells. Addition of Leukemia Inhibitory Factor (LIF) can also induce the efficient conversion of partial- or pre-iPS cells into fully pluripotent iPS cells via Nanog (Silva et al. 2008; Silva et al. 2009).

The 2i technology has also been used to establish ES cells from mouse strains for which it is difficult to produce germline-competent ES cells (Nichols et al. 2009) and to induce iPS cells from human and livestock species (Wang et al. 2011; Malaver-Ortega et al. 2012).

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Overview

• Completely defined, serum-free formulation promotes cell proliferation and maintains pluripotent state

• Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK, allowing establishment and long-term maintenance of germline-competent mouse ES and iPS cells

• Can be combined with Leukemia Inhibitory Factor (LIF) to induce the efficient conversion of partial- or pre-iPS mouse cells into fully pluripotent iPS cells

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Applications

• Establishment of mouse ES and iPS cell lines

• Long-term maintenance of pure ES or iPS cells

• Conversion of partial- or pre-iPS cells into fully pluripotent cells in the presence of LIF

Components

• 1 bottle (200 ml) of Cellartis mES/iPSC Culture Basal Medium

• 1 tube (200 µl) Cellartis 2i mES/iPSC Supplement

References

Malaver-Ortega, L. F. et al. The state of the art for pluripotent stem cells derivation in domestic ungulates. Theriogenology 78, 1749–1762 (2012).

Nichols, J. et al. Validated germline-competent embryonic stem cell lines from non-obese diabetic mice. Nat. Med. 15, 814–818 (2009).

Silva, J. et al. Promotion of reprogramming to ground state pluripotency by signal inhibition. PLoS Biol. 6, e253 (2008).

Silva, J. et al. Nanog is the gateway to the pluripotent ground state. Cell 138, 722–37 (2009).

Wang, W. et al. Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog. PNAS 108, 18283–18288 (2011).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.