Thermo Fisher Scientific HLA-DR Monoclonal Antibody (L243), APC-eFluor 780, eBioscience

Thermo Fisher Scientific HLA-DR Monoclonal Antibody (L243), APC-eFluor 780, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 2 publications

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The L243 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class II, HLA-DR.
HLA-DR is expressed on the surface of antigen-presenting cells (APC) such as B cells, monocytes, macrophages, dendritic cells, and activated T cells.
It plays a critical role in peptide presentation to CD4⁺ T lymphocytes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells
  • Recommended use: 5 µL (0.25 µg)/test (final volume 100 µL)
  • Cell number range: 10⁵–10⁸ cells/test

Fluorochrome Information:

• Excitation: 633–647 nm
• Emission: 780 nm
• Laser: Red Laser
• Light sensitivity: Protect from light (photo-induced oxidation)

Fixation Recommendations:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark.

Target Information

HLA-DR is a transmembrane glycoprotein consisting of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).

• Alpha chain: Encoded by 5 exons; lacks polymorphisms in peptide binding region.
• Beta chain: Contains polymorphisms determining peptide binding specificity.

Hundreds of DRB1 alleles have been described, and typing for these polymorphisms is routinely used in bone marrow and kidney transplantation.
HLA-DR is primarily expressed on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.

Function:
MHC class II molecules bind processed peptides and present them to T-helper cells, initiating immune responses.
HLA and MHC antibodies are crucial in Immunopeptidomics for neoantigen identification via LC-MS/MS.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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