Thermo Fisher Scientific CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), eBioscience
인간 CD90(Thy-1)을 인식하는 단클론 항체로, Flow Cytometry, WB, IHC 등에 사용 가능. eBio5E10 클론, Mouse IgG1, 비결합형. 고순도(>90%)로 제조되었으며, 세포 부착 및 신호전달 연구에 적합. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Immunohistochemistry (IHC) | - | 3 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 0.5 µg/test | 47 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio5E10 (5E10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864940 |
Additional Formats Available
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 786
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Custom conjugation available upon request
Product Specific Information
The eBio5E10 monoclonal antibody reacts with human CD90 (Thy-1), a 25–35 kDa receptor expressed on thymocytes, CD34⁺ prothymocytes, hematopoietic stem cells, neurons, and subsets of fetal liver, cord blood, and bone marrow cells.
CD90⁺CD34⁺ populations are enriched for cells capable of long-term culture. CD90 is involved in T-cell adhesion and signal transduction regulation.
Applications Reported:
This antibody is suitable for flow cytometry, immunoprecipitation, Western blotting, and frozen tissue immunohistology.
Applications Tested:
Tested by flow cytometric analysis of human erythroleukemia (HEL) cells. Recommended ≤0.5 µg/test (100 µL final volume). Optimal titration is advised for specific assays.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90 (Thy-1) is a GPI-linked glycoprotein of the Immunoglobulin superfamily, expressed in T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts.
It plays roles in cell-cell and cell-ligand interactions during synaptogenesis and neuronal development.
CD90 serves as a marker for various stem cells and axonal processes of mature neurons.
Structural studies of CD90 contributed to the foundation of the Immunoglobulin superfamily and the first biochemical description of a vertebrate GPI anchor.
CD90 dysfunction is associated with diseases such as nasopharyngeal carcinoma and thymoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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