Thermo Fisher Scientific Phospho-CDK2 (Thr14) Polyclonal Antibody
Human CDK2 Thr14 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Dot blot에 1:500 희석으로 사용 가능. Protein A 및 항원 친화 크로마토그래피로 정제됨. PBS(pH 7.4) 버퍼에 0.09% sodium azide 포함. 4°C 단기, -20°C 장기 보관 권장.
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Applications and Tested Dilution
- Dot blot (DB): 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Thr14 of human CDK2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.35 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10980562 |
Product Specific Information
This antibody is predicted to react with bovine, hamster, mouse, rat, and Xenopus based on sequence homology.
Target Information
CDK2/cyclinO is a member of the cyclin-dependent protein kinase family complexed to CyclinO. CyclinO, a specific protein isoform of the UNG gene, is localized exclusively in the nucleus and exhibits uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during the G1 phase and are turned over during the cell cycle. Cyclin A–cdk2 levels are maximal at the G1/S transition, with both cdk2 and cyclin A associated with DNA in the replication initiation complex. Rb protein acts as a substrate for cdk2–cyclin E and/or cdk2–cyclin A in vivo. Cdk2 is activated and localized to the nucleus during late G1, S, and G2 phases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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