Thermo Fisher Scientific Phospho-ERK1 (Thr202, Tyr205) Polyclonal Antibody
ERK1/2의 Thr202 및 Tyr205 인산화 상태를 검출하는 Rabbit Polyclonal Antibody. Western blot, ICC/IF, Dot blot에 적합. Human 반응성 및 Mouse, Rat, Dog에서도 예측 반응. 고순도 Protein A 및 항원 친화 크로마토그래피 정제, 연구용 전용.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Dot blot (DB) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T202/Y204 of human ERK1/2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10980423 |
Product Specific Information
This antibody is predicted to react with canine, mouse, and rat based on sequence homology.
Target Information
MAPK3 (ERK1) is a serine/threonine kinase that acts as an activator of p90, RSK, MSK, ELK1, and Stat3, and plays a key role in the MAPK signaling pathway. ERK1 regulates meiosis, mitosis, and post-mitotic functions in differentiated cells. It is activated by various stimuli such as growth factors, cytokines, and viral infections, leading to nuclear translocation and phosphorylation of nuclear targets. ERK1 is involved in cellular proliferation, differentiation, and cell cycle progression in response to extracellular signals. Multiple alternatively spliced transcript variants encoding different protein isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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