Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Blue 610-30S, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), NovaFluor Blue 610-30S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin, eFluor 450, FITC
• Functional Grade
• NovaFluor Blue 510–800, Red 700–755, UV 765, Violet 690–800, Yellow 590–810
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436–780
• Custom conjugation available

Product Specific Information

The OKT8 monoclonal antibody reacts with the human CD8a molecule (32–34 kDa), expressed as either CD8αβ heterodimer or CD8αα homodimer. CD8a is found on thymocytes, mature T cells, and NK cells. It binds MHC class I and associates with p56lck, playing a key role in T-cell development and activation.

Preliminary testing shows OKT8 does not compete with RPA-T8 or HIT8a clones for binding to human leukocytes, suggesting distinct epitopes.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of normal human peripheral blood cells at 5 µL (0.5 µg)/test (final volume 100 µL; 10⁵–10⁸ cells/test)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Product # C001T06F01) is required for optimal staining.
• Add 5 µL of CellBlox Plus per sample or per 100 µL cell suspension.
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescence minimizing spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin family. It acts as a co-receptor with the T cell receptor (TCR) for MHC class I molecules, facilitating T cell activation and signal transduction. CD8 exists as a CD8αβ heterodimer or CD8αα homodimer, expressed on T cells, NK cells, monocytes, and dendritic cells. Activation of CD8+ cytotoxic T lymphocytes (CTLs) is crucial for pathogen and tumor cell clearance, enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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