Thermo Fisher Scientific PRAS40 Polyclonal Antibody
PRAS40 단백질을 인식하는 Thermo Fisher Scientific의 토끼 다클론 항체. Western blot 및 IHC(파라핀 절편)에서 사용 가능하며, 인간, 생쥐, 랫트 반응성. 액상 형태, 항원 친화 크로마토그래피 정제, 고순도 제품으로 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 210–260 of Human PRAS40 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803056 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after various cell death signals.
AKT1S1 (also known as PRAS40) is a proline-rich substrate of the kinase AKT1 and plays a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia.
AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cycle, and is a negative regulator of autophagy.
Treatment with insulin-like growth factor-1 (IGF1) induces phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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