Thermo Fisher Scientific Rabbit anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 594
DyLight 594 형광으로 표지된 Rabbit anti-Sheep IgG(H+L) 2차 항체로, WB, IHC, ICC, Flow 등 다양한 실험에 사용 가능. 교차흡착 처리로 비특이적 반응 최소화. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 신뢰성 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Sheep IgG-heavy and light chain |
| Conjugate | DyLight™ 594 |
| Excitation / Emission Max | 587 / 614 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556636 |
| Target | IgG |
| Cross Adsorption | Against chicken, horse, human, mouse, and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to chicken, horse, human, mouse, and rat.
Target Information
Anti-Sheep secondary antibodies are affinity-purified antibodies with well-characterized specificity for ovine (sheep) immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, enabling use with various detection systems such as HRP, AP, or fluorescence. They also enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to achieve high specificity.
Additional Formats
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- Request custom conjugation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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