Thermo Fisher Scientific Rabbit anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
DyLight 550 형광 표지를 가진 Rabbit anti-Sheep IgG (H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 면역친화 크로마토그래피로 정제되어 높은 특이성과 낮은 교차반응성을 제공. 연구용으로 사용.
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Thermo Fisher Scientific Rabbit anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Sheep IgG-heavy and light chain |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556635 |
| Target | IgG |
| Cross Adsorption | Against chicken, horse, human, mouse, and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to chicken, horse, human, mouse, and rat.
Target Information
Anti-Sheep secondary antibodies are affinity-purified reagents with well-characterized specificity for ovine (sheep) immunoglobulins. They are useful for detection, sorting, or purification of sheep IgG targets.
Secondary antibodies enhance versatility and sensitivity by enabling various detection systems (e.g., HRP, AP, fluorescence) and providing signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
- DyLight 488
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- Request custom conjugation
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