Thermo Fisher Scientific Cytokeratin 4 Monoclonal Antibody (6B10)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:20

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6B10

Immunogen

Cytokeratin purified from human esophagus if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 4,uniProtId:P19013-1,ncbiNodeId:9606,antigenRange:1-520,antigenLength:520,antigenImageFileName:MA1-35558_Cytokeratin_4_P19013-1_House_mouse.svg,antigenImageFileNamePDP:MA1-35558_Cytokeratin_4_P19013-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

15 µg/mL

Storage buffer

tissue culture supernatant

Contains

10mM sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2134043

Product Specific Information

The antibody stains non-cornifying squamous epithelium and transitional epithelium of the bladder, cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands. In immunoblotting experiments the antibody was shown to recognize only cytokeratin no.4 (59 kDa).

Target Information

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between approximately40 kDa and approximately68 kDa and isoelectric pH between 4. 9-7. 8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.