Thermo Fisher Scientific TRIB1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 203-372 of human TRIB1 (NP_0794711) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRIB1,uniProtId:Q96RU8-1,ncbiNodeId:9606,antigenRange:203-372,antigenLength:372,antigenImageFileName:PA5-87662_TRIB1_Q96RU8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87662_TRIB1_Q96RU8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.35 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804331

Product Specific Information

Immunogen sequence: LGDLKLRKFV FSTEERTQLR LESLEDTHIM KGEDDALSDK HGCPAYVSPE ILNTTGTYSG KAADVWSLGV MLYTLLVGRY PFHDSDPSAL FSKIRRGQFC IPEHISPKAR CLIRSLLRRE PSERLTAPEI LLHPWFESVL EPGYIDSEIG TSDQIVPEYQ EDSDISSFFC; Positive Samples: U-87MG, BxPC-3, Raji

Target Information

TRIB1 also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities. Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.