Thermo Fisher Scientific MBP Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: Not specified
• Publications: View 1 publication

Immunohistochemistry (IHC)

• Tested Dilution: Not specified
• Publications: View 1 publication

Immunohistochemistry (Frozen) (IHC (F))

• Tested Dilution: 1:1,000–1:4,000
• Publications: None

Product Specifications

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution, keep aliquots at -20°C for higher stability, and at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.

Target Information

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP-related transcripts are also present in bone marrow and the immune system. These mRNAs arise from the long MBP gene (also called Golli-MBP) that contains 3 additional exons located upstream of the classic MBP exons.
Alternative splicing from the Golli and MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons, encoding hybrid proteins with N-terminal Golli sequence linked to MBP sequence. The second family of transcripts contains only MBP exons and produces well-characterized myelin basic proteins.
This complex gene structure is conserved among species, suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.