Thermo Fisher Scientific CD229 Monoclonal Antibody (HLy9.25), APC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

10 µL/1x10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HLy9.25

Immunogen

CD299-transfected 300.19 pre-B cell line if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD229 (Ly9),uniProtId:Q9HBG7-1,ncbiNodeId:9606,antigenRange:1-655,antigenLength:655,antigenImageFileName:MA1-10150_CD229_Ly9_Q9HBG7-1_House_mouse.svg,antigenImageFileNamePDP:MA1-10150_CD229_Ly9_Q9HBG7-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

View additional formats

• Unconjugated
• PE
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11155815

Product Specific Information

This antibody recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.

Target Information

CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.