Thermo Fisher Scientific CD229 Monoclonal Antibody (HLy9.25), APC
CD229/Ly9 단백질을 인식하는 마우스 단일클론 항체(HLy9.25)로, APC로 표지되어 T 및 B 세포 표면 발현 분석에 적합합니다. 인체 시료 반응성이 있으며, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 연구용으로만 사용 가능합니다.
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Applications
- Flow Cytometry (Flow): 10 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HLy9.25 |
| Immunogen | CD229-transfected 300.19 pre-B cell line |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11155815 |
Additional Formats
- Unconjugated: MA1-10149
- PE: MA1-10151
- Custom conjugation available upon request
Product Specific Information
This antibody recognizes an extracellular epitope of CD229 (Ly9), a 100–120 kDa cell surface glycoprotein expressed on T and B cells.
Target Information
CD229 (Ly9) is a cell surface receptor of the CD150 family, which also includes CD48 and CD224. These receptors regulate cytokine production and cytotoxicity of lymphocytes and NK cells.
High levels of CD229 are found on T and B cells, with expression increasing during maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets, and erythrocytes. CD229 has been reported on mouse monocytes and NK cells.
CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen-presenting B cells during antigen-dependent immune synapse formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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