Thermo Fisher Scientific SUMO1 Monoclonal Antibody (T.243.0)
SUMO1 단백질을 인식하는 고특이성 Rabbit Monoclonal Antibody (Clone T.243.0). Western blot, IHC, ICC, IP 등 다양한 응용 가능. 고순도 Affinity chromatography 정제, 안정적 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | T.243.0 |
| Immunogen | Synthetic peptide from the amino terminus of human SUMO-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 23 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10979860 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in cellular processes such as nuclear transport, DNA replication and repair, mitosis, and signal transduction. SUMO1 is involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are susceptible to polyubiquitination, signaling proteasomal degradation of modified proteins. SUMO1 may regulate genes involved in palate development, and mutations in the gene can result in non-syndromic orofacial cleft 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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