Thermo Fisher Scientific ATM Monoclonal Antibody (C.74.4)

Applications

• Western Blot (WB): 1:1,000
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Product Specifications

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene encodes a protein in the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins rather than lipids and has many downstream targets that act as cell-cycle regulators, including P53, Mdm2, BRCA1, and SMC1.

The ATM protein is responsible for repairing double-stranded DNA breaks caused by ionizing radiation and other mutagens. Its C-terminal region shows extensive homology to the catalytic domains of phosphatidylinositol 3-kinases. Studies indicate that ATM becomes autophosphorylated and upregulated upon exposure to ionizing radiation. AT cells are hypersensitive to such radiation, exhibit impaired DNA synthesis inhibition, and display delayed p53 induction. DNA damage activates ATM kinase, triggering a cascade of kinase reactions regulating cell cycle, apoptosis, and DNA damage repair. ATM has also been linked to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.