Thermo Fisher Scientific Human AURKA (Aurora A), His Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

His-tag

Molecular weight

49.9 kDa

Class

Recombinant

Type

Protein

Purity

85% by SDS-PAGE

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 50% glycerol, 4mM DTT, 150mM NaCl, 0.5mM EDTA, 0.05% Triton X-100

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Protein Form: Recombinant, Full Length

Target Information

AURKA (Aurora A) is a serine/threonine kinase that regulates mitosis by association in vivo with centrosomes. Overexpression of AURKA is observed in many types of cancer. Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment. Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.