Thermo Fisher Scientific Donkey anti-Sheep IgG (H+L) Secondary Antibody, AP
Thermo Fisher Scientific의 Donkey anti-Sheep IgG (H+L) AP 결합 2차 항체로, Sheep IgG 검출에 사용됩니다. Western blot, IHC, ELISA에 적합하며, 높은 특이성과 민감도를 제공합니다. Affinity chromatography로 정제되었으며, AP 효소로 결합되어 신호 증폭에 유리합니다.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Sheep |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C for short term; -20°C for long term. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534718 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Biotin conjugate
- FITC conjugate
- HRP conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
Each lot of antibody is validated for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-Sheep secondary antibodies are affinity-purified and exhibit well-characterized specificity for ovine (sheep) immunoglobulins. They are commonly used for detection, sorting, or purification of target antibodies.
Secondary antibodies provide flexibility across detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified through immunoaffinity chromatography, antibody fragmentation, or label conjugation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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