
Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin
Thermo Fisher Scientific의 Donkey anti-Rabbit IgG (H+L) Biotin 결합 항체는 고도로 교차흡착된 2차 항체로, Western blot, ICC/IF, Flow cytometry, ELISA에 적합합니다. 높은 특이성과 낮은 교차반응성을 제공하며, 안정적인 액상 형태로 장기 보관이 가능합니다.
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Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534713 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor 680
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- AP
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies provide versatility for various detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification. These antibodies are typically generated by immunizing the host animal with pooled rabbit immunoglobulins and can be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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