Thermo Fisher Scientific Cortactin Polyclonal Antibody
Rabbit polyclonal antibody against Cortactin for WB and IHC(F). Reacts with human, mouse, and rat. Lyophilized form, reconstitutable in DDW. Stored at -20°C. Suitable for actin cytoskeleton and cell motility studies.
- 카탈로그번호
- APZ-018-xxxxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Cortactin Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100 |
| Immunohistochemistry (Frozen) (IHC(F)) | 1:300 |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)KTSNIRANFENLAK, corresponding to amino acid residues 346–359 of mouse Cortactin, intracellular |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution:
- Add 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
- Ships as lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- After reconstitution, store at 4°C for up to 1 week.
- For long-term storage, aliquot and store at -20°C.
- Avoid multiple freeze/thaw cycles.
- Centrifuge antibody preparations before use (10,000 × g, 5 min).
Target Information
Cortactin (Cttn) is a ubiquitous actin-binding protein originally identified as a substrate for Src kinases. It plays a key role in actin cytoskeleton organization, cell shape, lamellipodia formation, and cell migration.
Cortactin is enriched in actin-rich peripheral structures and promotes actin network formation. It contains four major domains: N-terminal acidic (NTA), tandem repeats, proline-rich, and SH3 domains.
The NTA domain interacts with the Arp2/3 and WASP complexes at F-actin branches. Cortactin contributes to cell motility and invasion, particularly through invadopodia formation.
It is phosphorylated by Src family kinases at Y421, Y466, Y482, and by Erk family kinases at S405 and S418.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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