Thermo Fisher Scientific Cortactin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:300

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)KTSNIRANFENLAK, corresponding to amino acid residues 346-359 of mouse Cortactin, Intracellular if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cortactin,uniProtId:Q60598-1,ncbiNodeId:10090,antigenRange:346-359,antigenLength:546,antigenImageFileName:APZ-018-200UL_Cortactin_Q60598-1_Rabbit.svg,antigenImageFileNamePDP:APZ-018-200UL_Cortactin_Q60598-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Cortactin (Cttn) is a ubiquitous actin-binding protein that was originally identified as a substrate for Src. It contributes to the organization of the actin cytoskeleton and cell shape. Cortactin also plays a role in the formation of lamellipodia and cell migration. It is accumulated in peripheral, actin-enriched structures of cells suggesting that cortactin facilitates actin network formation. Cortactin has four major domains of interest the N-terminal acidic (NTA) and tandem repeats domains and the C-terminal proline-rich and SH3 Domains. NTA associates with the Arp2/3 and WASP complex at F-actin branches. Cortactin is involved in promoting cell motility and invasion, including a critical role in invadopodia, actin rich-subcellular protrusions associated with degradation of the ECM by cancer cells. Cortactin is phosphorylated by src family kinases at Y421, Y466, and Y482 and S405 and S418 that are phosphorylated by Erk family kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.