Takara RACE-ready cDNA: rat

Overview

• Adaptor-ligated and ready for RACE PCR
• Tissue-specific pool of double-stranded cDNA
• Obtain full-length cDNAs without constructing or screening a cDNA library
• Ideal template for Marathon cDNA amplification

Applications

• Rapid amplification of cDNA ends (RACE)
• Study tissue-specific gene expression
• Discover polymorphic forms of mRNA
• Obtain full-length copies of published cDNAs
• Characterize tissue-specific patterns of gene expression

Source

For relevant source information, please refer to the Certificate of Analysis accompanying each Marathon-Ready cDNA.

References

Borson, N. D., Salo, W. L. & Drewes, L. R.
A lock-docking oligo(dT) primer for 5′ and 3′ RACE PCR. PCR Methods Appl. 2, 144–148 (1992).

Chenchik, A., Diachenko, L., Moqadam, F., Tarabykin, V., Lukyanov, S., and Siebert, P. D.
Full-Length cDNA Cloning and Determination of mRNA 5′ and 3′ Ends by Amplification of Adaptor-Ligated cDNA. Biotechniques 21, 526–534 (1996).

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.