Takara Universal human cDNA

Overview

• Highly purified double-stranded cDNA
• Clone genes directly by PCR, rather than library screening
• Prepared from high-quality human tissues and cell lines
• Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

Applications

• Clone cDNAs without library screening
• Generate hybridization probes using gene-specific or degenerate primers
• Ideal for amplifying previously isolated, structurally related, or cross-species cDNAs

References

• Lee, C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239 (1988).
• Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246 (1989).
• Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149–158 (1990).
• Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57–61 (1990).
• Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67–74 (1989).

Additional Product Information

Please refer to the product’s Certificate of Analysis for details regarding storage conditions, product components, and technical specifications.
The Kit Components List is also available under the Documents tab.