Thermo Fisher Scientific MTCO1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody detects endogenous levels of total Cytochrome c Oxidase 1.

Target Information

Cytochrome c oxidase subunit I (CO1 or MTCO1) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV.
Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides.
Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA, while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded.
The cytochrome c oxidase family of enzymes has four redox centers: two hemes and two copper centers. In mitochondrial Complex IV, the two hemes are a and a3, and the two coppers are CuA and CuB. The two hemes and CuB are bound to subunit I.

Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. They hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.