Thermo Fisher Scientific CD16/CD32 Recombinant Rat Monoclonal Antibody (2.4G2)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Inhibition Assays (IA)

Assay-dependent

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2b, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2.4G2

Immunogen

Mouse BALB/c Macrophage J774 Cell Line. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD16/CD32,uniProtId:P08101-1,ncbiNodeId:10090,antigenRange:1-330,antigenLength:330,antigenImageFileName:MA5-51919_CD16CD32_P08101-1_Rat.svg,antigenImageFileNamePDP:MA5-51919_CD16CD32_P08101-1_Rat_PDP.jpeg,sortOrder:1},{targetFamily:CD16/CD32,uniProtId:P08508-1,ncbiNodeId:10090,antigenRange:1-261,antigenLength:261,antigenImageFileName:MA5-51919_CD16CD32_P08508-1_Rat.svg,antigenImageFileNamePDP:MA5-51919_CD16CD32_P08508-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

This antibody binds to an epitope on the extracellular domains of the mouse FcGammaIII and FcGammaII, thereby inhibiting their ability to bind antibodies via their Fc region. Also binding the FcGammaI receptor (CD64) via its Fc domain.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.