Thermo Fisher Scientific CD64 Monoclonal Antibody (10.1), PE-eFluor 610, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 3 publications

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high-affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and cytokine regulation. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated for use at 5 µL (0.25 µg)/test
• Cell Range: 10⁵–10⁸ cells/test

Fluorochrome Information:
PE-eFluor 610 can be excited with laser lines from 488–561 nm and emits at 607 nm. Recommended filter: 610/20 band pass (equivalent to PE-Texas Red). Ensure instrument compatibility.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.

Excitation/Emission: 488–561 nm / 607 nm
Compatible Lasers: Blue, Green, Yellow-Green

Target Information

CD64 (FCGR1A) encodes a high-affinity Fc-gamma receptor involved in immune response. It is one of three related gene family members on chromosome 1. Dysfunction of CD64 has been studied in cervical adenitis and tetrasomy 21.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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