Thermo Fisher Scientific Adeno-Associated Virus Capsid Protein Polyclonal Antibody, Biotin
Adeno-Associated Virus Capsid Protein에 특이적인 Biotin 결합 폴리클로날 항체로, Western blot, ELISA, Immunoprecipitation 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 안정적인 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids within region 200–250 on rat 5-hydroxytryptamine receptor 1A protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Proprietary buffer with 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Capsid protein self-assembles to form an icosahedral capsid with T=1 symmetry, approximately 22 nm in diameter, consisting of 60 copies of three size variants of the capsid protein VP1, VP2, and VP3 which differ in their N-terminus.
The capsid encapsulates the genomic ssDNA and binds to host cell heparan sulfate, using host ITGA5-ITGB1 as a coreceptor to attach to target cells.
This attachment induces virion internalization predominantly through clathrin-dependent endocytosis.
Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signals.
VP1 N-terminus may act as a lipolytic enzyme to breach the endosomal membrane during entry and contribute to viral transport to the nucleus.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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