Thermo Fisher Scientific iNOS Recombinant Rat Monoclonal Antibody (CXNFT)
iNOS 단백질 검출용 Recombinant Rat Monoclonal Antibody (Clone CXNFT). Western blot, IHC, ICC/IF, Flow Cytometry에 적합. 높은 특이성과 로트 간 일관성 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:4,000 |
| Flow Cytometry (Flow) | 0.125–1 µg/test |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rat / IgG2a, kappa |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | CXNFT |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Available Formats
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 647
- Custom conjugation available upon request
Product Specific Information
Recombinant Rat monoclonal antibodies are produced using in vitro expression systems. These antibodies are generated by cloning specific genes encoding the desired antibody into an expression vector, followed by in vitro expression.
Key advantages include improved specificity and consistent performance between lots.
It is recommended to titrate the antibody for optimal results in each assay.
Target Information
iNOS (Inducible Nitric Oxide Synthase) is an enzyme responsible for the production of nitric oxide (NO), a gaseous signaling molecule involved in vasorelaxation, neurotransmission, and immune response.
iNOS expression is inducible by lipopolysaccharides and cytokines such as IFN-γ, TNF, and IL-1/2. It catalyzes the oxidation of L-arginine to produce L-citrulline and NO.
Three isoforms exist: nNOS (neuronal, type I), iNOS (inducible, type II), and eNOS (endothelial, type III).
iNOS is found in macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Dysregulation of iNOS is associated with diseases such as achalasia and impotence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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