Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 12 publications 12 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 14 publications 14 publications

Flow Cytometry (Flow)

Assay-Dependent

View 14 publications 14 publications

Neutralization (Neu)

-

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

DECMA-1

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Biotin
• eFluor 660
• Functional Grade
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10671270

Product Specific Information

Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton.

Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers.

E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided.

The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells.

Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and immunocytochemistry.

Applications Tested: This DECMA-1 antibody has been tested by immunocytochemistry on formaldehyde fixed and permeabilized MDCK cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.