Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Alexa Fluor 488, eBioscience
Alexa Fluor 488으로 표지된 DECMA-1 클론의 CD324(E-Cadherin) 단클론 항체로, 인간·마우스·견 시료에서 반응합니다. 세포 간 접착 연구 및 암 진행 관련 연구에 적합하며, ICC/IF, IHC, Flow Cytometry 등에 사용 가능합니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Alexa Fluor 488
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 5 |
| Immunohistochemistry (IHC) | - | 12 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 3 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 14 |
| Flow Cytometry (Flow) | Assay-Dependent | 14 |
| Neutralization (Neu) | - | 3 |
| Miscellaneous PubMed (Misc) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DECMA-1 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10671270 |
Additional Formats
- Unconjugated
- Biotin
- eFluor 660
- Functional Grade
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The DECMA-1 monoclonal antibody recognizes mouse, human, and canine CD324 (E-cadherin), also known as epithelial cadherin or uvomorulin. E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion and connects to the cytoskeleton via catenins (α, β, γ) and actinins.
- Expressed in most epidermal cells, including melanocytes and keratinocytes
- Localized at intercellular boundaries of epithelial cells
- Plays a role in maintaining tissue integrity
- Loss of function implicated in cancer progression
- Sensitive to trypsin treatment — avoid exposure
The DECMA-1 antibody disrupts adhesion in human, mouse, and dog cells. It is suitable for flow cytometry, immunohistochemistry (frozen sections), and immunocytochemistry.
Tested Application:
Immunocytochemistry on formaldehyde-fixed and permeabilized MDCK cells ≤ 20 µg/mL
Careful titration recommended for optimal performance.
Optical Properties:
Excitation: 488 nm
Emission: 519 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered
Target Information
E-Cadherin (epithelial cadherin) is a calcium-dependent cell-cell adhesion glycoprotein with five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. Mutations are associated with various cancers (gastric, breast, colorectal, thyroid, ovarian). Loss of E-cadherin function correlates with increased proliferation, invasion, and metastasis.
- Mediates bacterial adhesion to mammalian cells
- Cytoplasmic domain required for internalization
- In humans, encoded by CDH1 gene on chromosome 16
- Plays a central role in tissue architecture and integrity
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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