Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Alexa Fluor 488

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• eFluor 660
• Functional Grade
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The DECMA-1 monoclonal antibody recognizes mouse, human, and canine CD324 (E-cadherin), also known as epithelial cadherin or uvomorulin. E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion and connects to the cytoskeleton via catenins (α, β, γ) and actinins.

• Expressed in most epidermal cells, including melanocytes and keratinocytes
• Localized at intercellular boundaries of epithelial cells
• Plays a role in maintaining tissue integrity
• Loss of function implicated in cancer progression
• Sensitive to trypsin treatment — avoid exposure

The DECMA-1 antibody disrupts adhesion in human, mouse, and dog cells. It is suitable for flow cytometry, immunohistochemistry (frozen sections), and immunocytochemistry.

Tested Application:
Immunocytochemistry on formaldehyde-fixed and permeabilized MDCK cells ≤ 20 µg/mL
Careful titration recommended for optimal performance.

Optical Properties:
Excitation: 488 nm
Emission: 519 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered

Target Information

E-Cadherin (epithelial cadherin) is a calcium-dependent cell-cell adhesion glycoprotein with five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. Mutations are associated with various cancers (gastric, breast, colorectal, thyroid, ovarian). Loss of E-cadherin function correlates with increased proliferation, invasion, and metastasis.

• Mediates bacterial adhesion to mammalian cells
• Cytoplasmic domain required for internalization
• In humans, encoded by CDH1 gene on chromosome 16
• Plays a central role in tissue architecture and integrity

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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