Thermo Fisher Scientific beta Catenin Monoclonal Antibody (15B8), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific beta Catenin Monoclonal Antibody (15B8), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, a member of the catenin family involved in cell adhesion through interaction with cadherins. Beta-catenin binds to the cytoplasmic tail of E-cadherin and participates in the canonical Wnt signaling pathway. Without Wnt stimulation, beta-catenin is phosphorylated and degraded; upon activation, it translocates to the nucleus and regulates gene expression with TCF and LEF transcription factors. Beta-catenin is expressed in thymocytes, T and B lymphocytes, and various tissues. This pathway is critical for T, B, and hematopoietic stem cell development.

Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.

Applications Tested: Pre-titrated and validated using Jurkat cells with the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended usage: 5 µL (0.25 µg) per test in 100 µL volume. Cell number range: 10⁵–10⁸ cells/test.

Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Beta-catenin is an adherens junction (AJ) protein essential for epithelial layer formation and maintenance. It interacts with E-cadherin and alpha-catenin, linking cell adhesion complexes to the actin cytoskeleton. Beta-catenin also associates with signaling complexes involving APC protein and microtubules, influencing morphogenesis and cell signaling. It is expressed in hair follicle cells and various epithelial tissues. Dysregulation of beta-catenin is implicated in cancers including colorectal, ovarian, breast, and skin tumors. In the nucleus, beta-catenin coactivates Lef/Tcf transcription factors, promoting expression of cyclin D and c-myc, which drive cell proliferation and tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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