Thermo Fisher Scientific beta Catenin Monoclonal Antibody (15B8), Alexa Fluor 488, eBioscience
Human 및 Mouse beta-catenin 검출용 Alexa Fluor 488 결합 단클론 항체. Wnt 신호전달 및 세포 접착 연구에 적합. Flow cytometry 및 IHC 등 다양한 응용 가능. 고순도 Affinity chromatography 정제, 4°C 암소 보관.
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Thermo Fisher Scientific beta Catenin Monoclonal Antibody (15B8), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 3 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 15B8 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10807094 |
Product Specific Information
The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, a member of the catenin family involved in cell adhesion through interaction with cadherins. Beta-catenin binds to the cytoplasmic tail of E-cadherin and participates in the canonical Wnt signaling pathway. Without Wnt stimulation, beta-catenin is phosphorylated and degraded; upon activation, it translocates to the nucleus and regulates gene expression with TCF and LEF transcription factors. Beta-catenin is expressed in thymocytes, T and B lymphocytes, and various tissues. This pathway is critical for T, B, and hematopoietic stem cell development.
Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated using Jurkat cells with the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00). Recommended usage: 5 µL (0.25 µg) per test in 100 µL volume. Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Beta-catenin is an adherens junction (AJ) protein essential for epithelial layer formation and maintenance. It interacts with E-cadherin and alpha-catenin, linking cell adhesion complexes to the actin cytoskeleton. Beta-catenin also associates with signaling complexes involving APC protein and microtubules, influencing morphogenesis and cell signaling. It is expressed in hair follicle cells and various epithelial tissues. Dysregulation of beta-catenin is implicated in cancers including colorectal, ovarian, breast, and skin tumors. In the nucleus, beta-catenin coactivates Lef/Tcf transcription factors, promoting expression of cyclin D and c-myc, which drive cell proliferation and tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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