Thermo Fisher Scientific UPF1 Polyclonal Antibody
UPF1 단백질을 인식하는 Thermo Fisher의 토끼 폴리클로날 항체. Western blot에 적합하며, 인간에 반응하고 마우스, 제브라피시, 드로소필라, 제노푸스에서도 예측 반응. 항원 친화 크로마토그래피로 정제된 액상 항체로, 1 mg/mL 농도. 연구용 한정.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human RENT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792475 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Zebrafish (98%), Drosophila (83%), Xenopus laevis (100%).
- Positive Control: 293T, A431, HeLa, HepG2.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Upf1 is an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons.
It functions as an ATP-dependent RNA helicase in the cytoplasm and is also a component of cytoplasmic P-bodies.
Upf1 phosphorylation mediates translation repression accompanying NMD, allowing mRNA accessibility to the NMD machinery.
Other NMD components, Upf2 and Upf3, are localized perinuclearly and nucleocytoplasmically, respectively.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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