Thermo Fisher Scientific MNAT1 Polyclonal Antibody
MNAT1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. E.coli 유래 재조합 단백질로부터 정제되었으며, 항원 친화 크로마토그래피로 정제됨. 세포주기 조절 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL | 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | – |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human MNAT1 recombinant protein (Position: L92–A278) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746805 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Progression through the cell cycle requires activation of a series of cyclin-dependent kinases (Cdks). The catalytic subunit Cdk2 is inactive alone and partially activated by binding regulatory cyclins such as cyclin D1, with full activation requiring phosphorylation at Thr160. The enzyme responsible for this phosphorylation, Cdk-activating kinase (CAK), comprises a catalytic subunit, a regulatory subunit (cyclin H), and a subunit of unknown function. The regulatory subunit Mat1 associates with the cyclin H–Cdk7 complex throughout the cell cycle, forming the active Cdk-cyclin complex. Cyclin H–Cdk7–Mat1 complexes also phosphorylate the carboxy terminus of RNA polymerase II, highlighting Mat1 as an essential assembly factor for Cdk-cyclin activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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