Thermo Fisher Scientific CDC6 Monoclonal Antibody (OTI1A4), TrueMAB
CDC6 단백질을 인식하는 Mouse Monoclonal Antibody로, Western Blot 및 IHC(Paraffin) 검증 완료. 인간 시료 반응성. 동결건조 형태로 제공되며, PBS 완충액과 트레할로스 포함. 연구용으로 DNA 복제 조절 단백질 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A4 |
| Immunogen | Full length human recombinant protein of human CDC6 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The CDC6 protein is highly similar to Saccharomyces cerevisiae Cdc6, a key regulator essential for the initiation of DNA replication.
It functions at the early stages of DNA replication and localizes in the nucleus during the G1 phase, translocating to the cytoplasm at the start of the S phase.
This translocation is regulated by phosphorylation through Cdks, and its transcription is controlled in response to mitogenic signals via E2F proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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