Thermo Fisher Scientific DDB1 Polyclonal Antibody
DDB1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 응용에 적합. Human, Mouse, Rat 시료에 반응하며, Affinity Chromatography로 정제됨. PBS buffer에 50% glycerol 포함, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1000–1140 of human DDB1 (NP_0019143) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3.63 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806374 |
Product Specific Information
- Immunogen sequence:
LGEFVNVFCH GSLVMQNLGE TSTPTQGSVL FGTVNGMIGL VTSLSESWYN LLLDMQNRLN KVIKSVGKIE HSFWRSFHTE RKTEPATGFI DGDLIESFLD ISRPKMQEVV ANLQYDDGSG MKREATADDL IKVVEELTRI H - Positive Samples: BT-474, Hep G2, NCI-H460, U-251MG, Jurkat, NIH/3T3, HeLa, Rat testis
- Cellular Location: Cytoplasm, Nucleus
Target Information
The DNA damage-binding protein (DDB) from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, purified human DDB protein was injected into XPE cells (Keeney et al., 1994).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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