Thermo Fisher Scientific HDAC9 (internal) Polyclonal Antibody
HDAC9 단백질의 내부 영역에 특이적으로 결합하는 Rabbit Polyclonal 항체. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 친화 크로마토그래피로 정제되었으며, 인체 반응성이 확인됨. 연구용으로 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-HDAC9 affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to the internal region of human HDAC9 protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- BLAST analysis indicates 100% sequence homology with human HDAC9.
- Cross-reactivity with HDAC9 from other species has not been determined.
Target Information
DNA associates with histones and nuclear proteins to form chromatin. Chromatin remodeling is essential for transcriptional regulation, and histone acetylation plays a key role in this process. Acetylation alters nucleosomal conformation, increasing DNA accessibility to transcription factors.
Histone deacetylases (HDACs) reverse this process, leading to transcriptional silencing. HDAC9 and its isoform HDAC9a both contain catalytic domains responsible for deacetylase activity and repress MEF2-mediated transcription. These proteins are expressed in brain, skeletal muscle, kidney, placenta, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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