Thermo Fisher Scientific IgG4 (Ig Heavy Constant Gamma 4) Recombinant Rabbit Monoclonal Antibody (IGHG4, 2042R)
Recombinant Rabbit Monoclonal Antibody targeting human IgG4 constant region. Validated for IHC (paraffin) and peptide array applications. Produced in HEK293 cells, purified by Protein A, unconjugated liquid form. Stable at -20°C or -80°C, suitabl...
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Peptide Array (Array)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | IGHG4, 2042R |
| Immunogen | Recombinant full-length human IGHG4 protein (Human IgG4 Heavy Chain, UniProt ID: P01861-2, Antigen range: 1–396) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry on PFA-fixed tissues:
- Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The constant regions (C regions) of immunoglobulins are sequences beyond the variable regions, present in both heavy and light chains.
These regions:
- Contain carbohydrate attachment sites.
- Hold variable regions together via disulfide bonds.
- Facilitate antigen interaction by increasing arm rotation.
Studies indicate that patients with recurrent respiratory tract infections often exhibit low IgG4 concentrations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(본 제품 관련 항원 구조 이미지: Human IgG4 Heavy Chain, UniProt ID: P01861-2)
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