Thermo Fisher Scientific FAK Polyclonal Antibody
FAK 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. 높은 특이성과 재현성을 제공하며, 세포 부착 및 신호전달 연구에 활용됩니다.
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Thermo Fisher Scientific FAK Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:800 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | View 1 publication |
| Immunoprecipitation (IP) | 1:50 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Non-human primate, Pig, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues surrounding amino acid 710 of human FAK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 31 µg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10988708 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other proteins.
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle.
It has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors.
Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase.
In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro, the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site-directed mutagenesis.
Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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