Thermo Fisher Scientific Human Alpha-1-Antitrypsin Polyclonal Antibody
Human alpha-1-antitrypsin을 인식하는 Thermo Fisher Scientific의 goat polyclonal 항체. Western blot, IHC, ICC/IF, ELISA에 적합. 항원 친화 크로마토그래피로 정제된 액상 항체로, PBS buffer에 0.09% sodium azide 포함. 연구용으로 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 – 1:30,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 – 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200 – 1:2,000 |
| ELISA | 1:1,000 – 1:30,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Goats were immunized with purified human alpha-1-antitrypsin derived from serum or plasma. The antibody was purified by antigen affinity chromatography using antigen-coupled agarose beads. Antibody concentration was determined using an extinction coefficient: an absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Target Information
Alpha-1-antitrypsin (Alpha-1-AT) is synthesized in the liver and functions as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which are released during inflammatory reactions in the lung.
Deficiency of alpha-1-AT can lead to chronic obstructive lung disease (emphysema) and, less frequently, hepatic cirrhosis in infants and respiratory distress in newborns.
Alpha-1-AT levels increase as an acute-phase response to tissue necrosis and inflammation and are elevated in conditions such as rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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