Thermo Fisher Scientific CD22/BL-CAM (B-Cell Marker) Monoclonal Antibody (BLCAM/1796)
CD22(BL-CAM) 단백질을 표적하는 mouse monoclonal antibody로, B세포 표지 연구에 적합합니다. WB, ICC/IF, Flow, ELISA 등 다양한 응용에 사용 가능하며, 200 µg/mL 농도의 액상 형태로 제공됩니다. Human 시료 반응성이 있으며, 4°C에서 보관합니다.
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Thermo Fisher Scientific CD22/BL-CAM (B-Cell Marker) Monoclonal Antibody (BLCAM/1796)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| ELISA | 2–4 µg/mL |
| Peptide Array | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BLCAM/1796 |
| Immunogen | Recombinant fragment of human CD22 protein (around aa 52–178) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with a molecular weight of 130–140 kDa. It is expressed in both the cytoplasm and membrane of B-lymphocytes and appears early in B-cell differentiation, similar to CD19.
CD22 membrane expression is limited to mature B cells (CD22+) and plasma cells (CD22−), making it useful for phenotyping mature leukemias. It is strongly expressed in hairy cell leukemia and positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
CD22 preferentially binds to α2,6-linked sialic acid and participates in B-cell receptor signaling regulation through tyrosine phosphorylation and interaction with Src family kinases. It can also act as an inhibitory receptor by recruiting cytoplasmic phosphatases that dephosphorylate signaling molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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