Thermo Fisher Scientific CD22/BL-CAM Monoclonal Antibody (BLCAM, 1795)
인간 CD22(BL-CAM) 단백질을 인식하는 마우스 단클론 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능합니다. Protein A/G로 정제된 액상 형태이며, 4°C에서 보관합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BLCAM, 1795 |
| Immunogen | Recombinant fragment (around aa52–178) of human BL-CAM, CD22 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD22 (BL-CAM) is a type I integral membrane glycoprotein with a molecular weight of 130–140 kDa. It is expressed in both the cytoplasm and cell membrane of B-lymphocytes. CD22 antigen appears early in B-cell differentiation, similar to CD19. Its membrane expression is limited to mature B cells (CD22⁺) and absent in plasma cells (CD22⁻), making it useful in phenotyping mature leukemias. CD22 is strongly expressed in hairy cell leukemia and is also positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
CD22 preferentially binds to α2,6-linked sialic acid. The recognition site can be masked by cis interactions on the same cell surface. Upon ligand-induced tyrosine phosphorylation, CD22 participates in regulation of B-cell antigen receptor signaling, acting as both a positive and inhibitory regulator through interactions with Src family kinases and cytoplasmic phosphatases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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