Thermo Fisher Scientific APH1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Hamster, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, KLH conjugated, corresponding to residues C (245) R R Q E D S R V M V Y S A L R I P P E D (265) of human APH-1aL/CT. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:APH1,uniProtId:Q96BI3-1,ncbiNodeId:9606,antigenRange:246-265,antigenLength:265,antigenImageFileName:PA1-2010_APH1_Q96BI3-1_Rabbit.svg,antigenImageFileNamePDP:PA1-2010_APH1_Q96BI3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.69 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.005% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2227105

Product Specific Information

PA1-2010 detects APH-1 in human and mouse samples.

PA1-2010 has been successfully used in immunofluorescent, immunoprecipitation, and Western blot procedures. By Western blot, this antibody detects a ~28 kDa protein representing APH-1 in mouse brain and kidney extracts.

The PA1-2010 immunogen is a Synthetic peptide, KLH conjugated, corresponding to residues C (245) R R Q E D S R V M V Y S A L R I P P E D (265) of the C-Terminal domain of the long isoform of APH-1a. This polyclonal antibody has been referred to as, O2C2 in the reference listed above.

Target Information

APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.