Thermo Fisher Scientific IgM (Immunoglobulin Mu Heavy Chain) Recombinant Rabbit Monoclonal Antibody (IGHM, 2559R)
Recombinant rabbit monoclonal antibody targeting human IgM heavy chain. Suitable for IHC (paraffin). Expressed in HEK293 cells, purified by Protein A/G. Supplied as liquid, 1 mg/mL in PBS, preservative-free. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | IGHM, 2559R |
| Immunogen | Heavy chain of human IgM |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry on PFA-fixed tissues, incubate the antibody for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
IgM is expressed intracellularly during early stages of B lymphopoiesis and later on the surface of more mature B cells in the bone marrow and peripheral B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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