Thermo Fisher Scientific BMI-1 Recombinant Rabbit Monoclonal Antibody (JJ093-3)
BMI-1 단백질을 표적으로 하는 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF에 적합하며 높은 특이성과 재현성을 제공. Protein A로 정제된 액상 형태로, 장기 보관 시 -20°C에서 안정적. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ093-3 |
| Immunogen | Synthetic peptide within Human Bmi1 aa 282–326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809763 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex, which maintains the transcriptionally repressive state of many genes, including Hox genes, throughout development.
The PRC1 complex functions via chromatin remodeling and histone modification, mediating monoubiquitination of histone H2A at Lys-119, leading to heritable changes in chromatin expressibility.
Within the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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