Thermo Fisher Scientific Phospho-c-Raf (Ser296) Polyclonal Antibody
Ser296 인산화된 c-Raf 단백질을 인식하는 Rabbit Polyclonal Antibody. Human 반응성, Protein A 및 항원 친화 크로마토그래피로 정제됨. 단기 4°C, 장기 -20°C 보관. 세포 신호전달 및 MAPK 경로 연구에 적합.
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Applications
Dot blot (DB)
- Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S296 of human RAF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A, Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979752 |
Product Specific Information
This antibody is predicted to react with bovine, mouse, and rat based on sequence homology.
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) that functions downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which in turn activate ERK1 and ERK2. Activated ERKs regulate gene expression related to cell division, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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