Thermo Fisher Scientific NME4 Monoclonal Antibody (OTI4F10), TrueMAB
NME4 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC, Flow Cytometry에 적합. HEK293T 세포에서 발현된 full-length human recombinant protein을 면역원으로 사용. 고순도 친화 크로마토그래피 정제 및 동결건조 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F10 |
| Immunogen | Full length human recombinant protein of human NME4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
NME4 is a member of the protein kinase family, enzymes that transfer phosphate groups from donors such as ATP to acceptor amino acids in substrate proteins.
These kinases mediate signal transduction in eukaryotic cells, regulating metabolism, transcription, cell cycle, cytoskeletal organization, apoptosis, and differentiation.
With over 500 gene products, the protein kinase family is one of the largest in eukaryotes, classified into 8 major groups based on catalytic domain sequences.
The AGC kinase group includes 63 kinases such as PKA, PKG, PKC, RAC/Akt, ARK, and RSK families.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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