Thermo Fisher Scientific Human TGF-beta 3 Recombinant Protein

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

-

Miscellaneous PubMed (Misc)

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View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Not Applicable

Expression System

E. coli

Amino acid sequence

MALDTNYCFR NLEENCCVRP LYIDFRQDLG WKWVHEPKGY YANFCSGPCP YLRSADTTHS TVLGLYNTLN PEASASPCCV PQDLEPLTIL YYVGRTPKVE QLSNMVVKSC KCS

Molecular weight

12.9 kDa

Class

Recombinant

Type

Protein

Purity

≥95%

Endotoxin concentration

≤0.1 EU/µg

Activity

ED50 ≤1 ng/mL; determined by the dose-dependent inhibition of HT-2 cell proliferation induced by IL-4.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

purified

Storage buffer

0.01M acetic acid with 20% ethanol

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Recombinant human TGF-beta3 is a non-glycosylated, disulfide-linked homodimer, containing two 112 amino acid chains.

Target Information

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.