Thermo Fisher Scientific Human HTRA1 Recombinant Protein

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

-

Product Specifications

Expression System

E. coli

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

RP-77541 is a truncated, recombinant human HtrA1 protein expressed in E. coli; amino acids 141-480 of full-length HtrA1 with a C-terminal 6x His-tag,containing the catalytic and PDZ domain.

RP-77541 may be used as a control with corresponding anti-HtrA1 antibodies or in an enzymatic assay. RP-77541 appears as a major protein of about 37 kDa in SDS-PAGE.

Target Information

HtrA1, a 51KDa protein, is a member of highly conserved mammalian HtrA serine protease family. It is a human homolog of bacterial HtrA and consists of a secretory signal sequence, an IGFBP-binding domain and a Kazal-type serine-protease inhibitor domain at the N-terminus and a highly conserved trypsin-like serine/protease domain and a PDZ domain at the C-terminus. It plays a major role in the regulation of cell growth. HtrA1 inhibits signaling mediated by TGF-beta family members and regulates many physiological processes. HtrA1 is up-regulated in placenta during pregnancy and plays an important role in implantation and placental physiology. It regulates the availability of IGFs by cleaving IGF-binding proteins. It acts as a tumor suppressor and is down-regulated in ovarian cancers and melanomas. Its role has been implicated in osteoarthritis suggesting a therapeutic intervention. Northern blot analysis detected high expression in placenta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.